VIC激發(fā)波長和發(fā)射波長

VIC激發(fā)波長和發(fā)射波長

VIC是一種熒光染料，初由Thermofisher 開發(fā)腻要。VIC的分子量為1023.38复罐，VIC激發(fā)波長(Ex)為526nm，發(fā)射波長(Em)為543nm雄家。VIC發(fā)射在可見光譜的綠黃色部分。用于在5′端( 可作為探針在各種實時熒光 PCR胀滚、雜交和基于熒光的遺傳分析中應(yīng)用 )熒光標(biāo)記寡核苷酸趟济。VIC磷酰胺化物是將VIC標(biāo)簽摻入寡核苷酸中方便的構(gòu)建塊。

Figure 1：VIC激發(fā)波長和發(fā)射波長的光譜圖

VIC is a fluorescent dye that was originally developed by Applied Biosystems (now, Thermofisher). VIC emits in the green-yellow part of the visible spectrum. It is used to fluorescently label oligonucleotides at the 5’-end (for use as probes in a variety of real-time PCR, hybridization, and fluorescence-based genetic analysis applications). VIC phosphoramidite is the most convenient building block to incorporate VIC tag into an oligonucleotide.

Molecular weight：1023.38

Excitation：526nm

Emission：543nm

Figure 2. Chemical structure for 5-VIC phosphoramidite.

上海峰志儀器有限公司代理銷售美國路陽各種款式熒光蛋白觀測燈咽笼，有LUYOR-3280手電筒式熒光蛋白觀察手電筒顷编、LUYOR-3415手持式雙波長熒光蛋白激發(fā)光源、LUYOR-3421臺式熒光蛋白激發(fā)光源剑刑，能夠滿足觀察熒光蛋白在模式動物媳纬、植物葉片、種子施掏、愈傷等上的表達钮惠。也能激發(fā)各種熒光染料發(fā)光。